منابع مشابه
Anti-DNA antibodies bind to DNase I
Polyspecificity is a well-known property of the anti-DNA antibodies produced by autoimmune animals. In our search for antigen targets of anti-DNA antibodies within tissue extracts, we identified a 32-kD polypeptide that was recognized by a large panel of anti-DNA antibodies. Direct sequencing of this protein disclosed its identity with DNase I. 22 monoclonal anti-DNA antibodies bound to DNase I...
متن کاملActinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure.
Two DNA hexadecamers containing one central 5'-GC-3' base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from the actinomycin intercalation site in a molecule of sequence d[ATATATAGCTATATAT]...
متن کاملShotgun DNA sequencing using cloned DNase I-generated fragments
A method for DNA sequencing has been developed that utilises libraries of cloned randomly-fragmented DNA. The DNA to be sequenced is first subjected to limit attach by a non-specific endonuclease (DNase I in the presence of Mn++), fractionated by size and cloned in a single-stranded phage vector. Clones are then picked at random and used to provide a template for sequencing by the dideoxynucleo...
متن کاملMelting of branched RNA molecules.
In this Letter we show that the melting thermodynamics of RNA molecules is very sensitive to the branching geometry. We find that, when pairing interactions are described by a Gō model, unbranched RNA molecules with a linear geometry melt via a conventional continuous phase transition with classical exponents while RNA molecules with the branching geometry of a Cayley tree, with coordination nu...
متن کاملDNaseR: DNase I footprinting analysis of DNase-seq data
The combination of DNase I digestion and high-throughput sequencing (DNaseseq) has been used recently to map chromatin accessibility in a given tissue or cell type on a genome-wide scale (Song and Crawford, 2010). In addition to DNase I hypersensitive sites (DHSs), short regions of protected nucleotides known as footprints can be detected using a technique known as ”digital genomic footprinting...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)30012-2